Methods
-Try out concentration of the compount, ICG, SH, light source etc. that works fine and give an absorbance of approx. 1.
-Check [nm], [Darkline] and [Baseline]
-Dilute the solution for the standard curve.
For example if the last concentration in 30mmol and 5 solution are made. You have to prepare 6 , 12 , 18 , 24 and 30mmol.
-Complete the dialog <Edit the method> and [add Method]. The method will appear in the methods window, with a line for each concentration.
-Now insert the cuvette with the specific concentration and select the line.
The messurement takes place and the absorbance will be stored
-If everything is ok, you can save the method and it’s values.
The fitting curve is determined by numpy’s polyfit and the r2_score and displayed.